Lectin microarray profiling of metastatic breast cancers

Fry, S., Afrough, B., Lomax-Browne, H., Timms, J.F., Velentzis, L.S. and Leathem, A. 2011. Lectin microarray profiling of metastatic breast cancers. Glycobiology. 21 (8), pp. 1060-1070. https://doi.org/10.1093/glycob/cwr045

TitleLectin microarray profiling of metastatic breast cancers
AuthorsFry, S., Afrough, B., Lomax-Browne, H., Timms, J.F., Velentzis, L.S. and Leathem, A.
Abstract

Altered protein glycosylation compared to the disease-free state is a universal feature of cancer cells. It has long been established that distinct glycan structures are associated with specific forms of cancer, but far less is known about the complete array of glycans associated with certain tumours. The cancer glycome has great potential as a source of biomarkers, but progress in this field has been hindered by a lack of available techniques for the elucidation of disease associated glycosylation. In the present study, lectin microarrays consisting of 45 lectins with different binding preferences covering N- and O-linked glycans were coupled with evanescent-field activated fluorescent detection in the glycomic analysis of primary breast tumours and the serum and urine of patients with metastatic breast cancer. A single 50 μm section of a primary breast tumour or less than 1 μL of breast cancer patient serum or urine was sufficient to detect glycosylation alterations associated with metastatic breast cancer, as inferred from lectin binding patterns. The high-throughput, sensitive and relatively simple nature of the simultaneous analysis of N- and O-linked glycosylation following minimal sample preparation and without the need for protein deglycosylation makes the lectin microarray analysis described a valuable tool for discovery phase glycomic profiling.

JournalGlycobiology
Journal citation21 (8), pp. 1060-1070
ISSN0959-6658
Year2011
PublisherOxford University Press
Digital Object Identifier (DOI)https://doi.org/10.1093/glycob/cwr045
Publication dates
Published2011

Related outputs

Serum IgA1 shows increased levels of α 2,6-linked sialic acid in breast cancer
Lomax-Browne, Hannah J., Robertson, Claire, Antonopoulos, Aristotelis, Leathem, Anthony J. C., Haslam, Stuart M., Dell, Anne and Dwek, M. 2019. Serum IgA1 shows increased levels of α 2,6-linked sialic acid in breast cancer. Interface Focus. 9 (2), p. 20180079 20180079. https://doi.org/10.1098/rsfs.2018.0079

Cellular glycosylation affects Herceptin binding and sensitivity of breast cancer cells to doxorubicin and growth factors
Peiris, D., Spector, A.F., Lomax-Browne, H., Azimi, T., Ramesh, B., Loizidou, M., Welch, H. and Dwek, M. 2017. Cellular glycosylation affects Herceptin binding and sensitivity of breast cancer cells to doxorubicin and growth factors. Scientific Reports. 7 43006. https://doi.org/10.1038/srep43006

Cadherin-5: a biomarker for metastatic breast cancer with optimum efficacy in oestrogen receptor-positive breast cancers with vascular invasion
Fry, S., Robertson, C.E., Swann, R. and Dwek, M. 2016. Cadherin-5: a biomarker for metastatic breast cancer with optimum efficacy in oestrogen receptor-positive breast cancers with vascular invasion. British Journal of Cancer. 114, pp. 1019-1026. https://doi.org/10.1038/bjc.2016.66

A targeted glycoproteomic approach identifies cadherin-5 as a novel biomarker of metastatic breast cancer
Fry, S., Sinclair, J., Timms, J.F., Leathem, A. and Dwek, M. 2013. A targeted glycoproteomic approach identifies cadherin-5 as a novel biomarker of metastatic breast cancer. Cancer Letters. 328 (2), pp. 335-344. https://doi.org/10.1016/j.canlet.2012.10.011

The DietCompLyf study: a prospective cohort study of breast cancer survival and phytoestrogen consumption
Swann, R., Perkins, K.A., Velentzis, L.S., Ciria, C., Dutton, S., Mulligan, A.A., Woodside, J., Cantwell, M.M., Leathem, A., Robertson, C.E. and Dwek, M. 2013. The DietCompLyf study: a prospective cohort study of breast cancer survival and phytoestrogen consumption. Maturitas. 75 (3), pp. 232-240. https://doi.org/10.1016/j.maturitas.2013.03.018

The DietCompLyf study: a prospective longitudinal study of breast cancer survival
Swann, R., Perkins, A., Velentzis, L.S., Mulligan, A.M., Woodside, J., Cantwell, M.M., Dutton, S., Leathem, A., Robertson, C.E. and Dwek, M. 2012. The DietCompLyf study: a prospective longitudinal study of breast cancer survival. European Journal of Cancer. 48 (5), p. S216. https://doi.org/10.1016/S0959-8049(12)71525-3

DietCompLyf study: a multi-centre UK study on breast cancer. What are the dietary and lifestyle changes following diagnosis?
Perkins, A., Swann, R., Woodside, J., Robertson, C.E., Dutton, S., Mulligan, A.M., Velentzis, L.S., Keshtgar, M.R., Leathem, A. and Dwek, M. 2012. DietCompLyf study: a multi-centre UK study on breast cancer. What are the dietary and lifestyle changes following diagnosis? European Journal of Cancer. 48 (5), p. S282. https://doi.org/10.1016/S0959-8049(12)71766-5

Genome-wide association analysis identifies three new breast cancer susceptibility loci
Ghoussaini, M., Fletcher, O., Michailidou, K., Turnbull, C., Schmidt, M.K., Dicks, E., Dennis, J., Wang, Q., Humphreys, M.K., Luccarini, C., Baynes, C., Conroy, D., Maranian, M., Ahmed, S., Driver, K., Johnson, N., Orr, N., Dos Santos Silva, I., Waisfisz, Q., Meijers-Heijboer, H., Uitterlinden, A.G., Rivadeneira, F., Hall, P., Czene, K., Irwanto, A., Liu, J., Nevanlinna, H., Aittomaki, K., Blomqvist, C., Meindl, A., Schmutzler, R.K., Muller-Myhsok, B., Lichtner, P., Chang-Claude, J., Hein, R., Nickels, S., Flesch-Janys, D., Tsimiklis, H., Makalic, E., Schmidt, D., Bui, M., Hopper, J.L., Apicella, C., Park, D.J., Southey, M.C., Hunter, D.J., Channock, S.J., Broeks, A., Verhoef, S., Hogervorst, F.B.L., Fasching, P.A., Lux, M.P., Beckmann, M.W., Ekici, A.B., Sawyer, E., Tomlinson, I., Kerin, M.J., Marme, F., Schneeweiss, A., Sohn, C., Burwinkel, B., Guenel, P., Truong, T., Cordina-Duverger, E., Menegaux, F., Bojesen, S.E., Nordestgaard, B.G., Nielsen, S.F., Flyger, H., Milne, R.L., Alonso, M.R., Gonzalez-Neira, A., Benitez, J., Anton-Culver, H., Ziogas, A., Bernstein, L., Clarke Dur, C., Brenner, H., Muller, H., Arndt, V., Stegmaier, C., Justenhoven, C., Brauch, H., Brüning, T., Wang-Gohrke, S., Eilber, U., Dörk, T., Schürmann, P., Bremer, M., Hillemanns, P., Bogdanova, N.V., Antonenkova, N.N., Rogov, Y.I., Karstens, J.H., Bermisheva, M., Prokofieva, D., Khusnutdinova, E., Lindblom, A., Margolin, S., Mannermaa, A., Kataja, V., Kosma, V.-M., Hartikainen, J.M., Lambrechts, D., Yesilyurt, B.T., Floris, G., Leunen, K., Manoukian, S., Bonanni, B., Fortuzzi, S., Peterlongo, P., Couch, F.J., Wang, X., Stevens, K.N., Lee, A., Giles, G.G., Baglietto, L., Severi, G., McLean, C.A., Grenaker Alnaes, G., Kristensen, V., Borrensen-Dale, A.-L., John, E.M., Miron, A., Winqvist, R., Pylkas, K., Jukkola-Vuorinen, A., Kauppila, S., Andrulis, I.L., Glendon, G., Mulligan, A.M., Devilee, P., van Asperen, C.J., Tollenaar, R.A.E.M., Seynaeve, C., Figueroa, J.D., Garcia-Closas, M., Brinton, L., Lissowska, J., Hooning, M.J., Hollestelle, A., Oldenburg, R.A., van den Ouweland, A.M.W., Cox, A., Reed, M.W.R., Shah, M., Jakubowska, A., Lubinski, J., Jaworska, K., Durda, K., Jones, M., Schoemaker, M.J., Ashworth, A., Swerdlow, A.J., Beesley, J., Chen, X., Muir, K., Lophatananon, A., Rattanamongkongul, S., Chaiwerawattana, A., Kang, D., Yoo, K.Y., Noh, D.Y., Shen, C.Y., Yu, J.C., Wu, P.E., Hsiung, C.N., Perkins, A., Swann, R., Velentzis, L.S., Eccles, D.M., Tapper, W.J., Gerty, S.M., Graham, N.J., Ponder, B.A.J., Chenevix-Trench, G., Pharoah, P.D.P., Lathrop, M., Dunning, A.M., Rahman, N., Peto, J. and Easton, D.F. 2012. Genome-wide association analysis identifies three new breast cancer susceptibility loci. Nature Genetics. 44 (3), pp. 312-318. https://doi.org/10.1038/ng.1049

Lectin array based strategies for identifying metastasis-associated changes in glycosylation
Fry, S., Afrough, B., Leathem, A. and Dwek, M. 2012. Lectin array based strategies for identifying metastasis-associated changes in glycosylation. Methods in Molecular Biology. 878, pp. 267-272. https://doi.org/10.1007/978-1-61779-854-2_18

Association of serum anti-Tn IgM with breast cancer recurrence
Afrough, B., Fry, S., Lomax-Browne, H., Perkins, A., Leathem, A. and Dwek, M. 2011. Association of serum anti-Tn IgM with breast cancer recurrence. Immunology. 135 (Supp.1), p. 153. https://doi.org/10.1111/j.1365-2567.2011.03534.x

Significant changes in dietary intake and supplement use after breast cancer diagnosis in a UK multicentre study
Velentzis, L.S., Keshtgar, M.R., Woodside, J., Leathem, A., Titcomb, A., Perkins, A., Mazurowska, M., Anderson, V., Wardell, K. and Cantwell, M.M. 2011. Significant changes in dietary intake and supplement use after breast cancer diagnosis in a UK multicentre study. Breast Cancer Research and Treatment. 128 (2), pp. 473-482. https://doi.org/10.1007/s10549-010-1238-8

Abstract B76 - An overview of the DietCompLyf study - a multicentre UK study to evaluate the role of diet, lifestyle and complementary medicine use on breast cancer recurrence
Swann, R., Perkins, A., Dahya, P., Woodside, J., Dutton, S., Robertson, C.E., Velentzis, L.S., Leathem, A. and Dwek, M. 2011. Abstract B76 - An overview of the DietCompLyf study - a multicentre UK study to evaluate the role of diet, lifestyle and complementary medicine use on breast cancer recurrence. National Cancer Research Institute (NCRI) Cancer Conference 2011. BT Convention Centre, Liverpool, UK 06 - 09 Nov 2011

Abstract LB14 - Lectin microarray profilling of metastatic breast cancers
Fry, S., Afrough, B., Lomax-Browne, H., Timms, J.F., Velentzis, L.S. and Dwek, M. 2011. Abstract LB14 - Lectin microarray profilling of metastatic breast cancers. National Cancer Research Institute (NCRI) Cancer Conference 2011. BT Convention Centre, Liverpool, UK 06 - 09 Nov 2011

ProSim: development of a user-friendly molecular modelling package
Heindl, H., Kiss, T., Terstyanszky, G., Weingarten, N., Afrough, B. and Greenwell, P. 2010. ProSim: development of a user-friendly molecular modelling package. in: Hicks, M.G. and Kettner, C. (ed.) Proceedings of the International Beilstein Symposium on Glyco-Bioinformatics: Bits ‘n’ Bytes of Sugars, October 4th – 8th, 2009, Potsdam, Germany Beilstein-Institut zur Fo¨rderung der Chemischen Wissenschaften. pp. 61-85

A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of prostate specific antigen (PSA) in patients with prostate cancer compared with benign prostatic hyperplasia
Dwek, M., Jenks, A. and Leathem, A. 2010. A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of prostate specific antigen (PSA) in patients with prostate cancer compared with benign prostatic hyperplasia. Clinica Chimica Acta. 411 (23-24), pp. 1935-1939. https://doi.org/10.1016/j.cca.2010.08.009

UEA-1 enables discrimination between prostate specific antigen from patients with prostate cancer and benign prostatic hyperplasia
Dwek, M., Jenks, A. and Leathem, A. 2009. UEA-1 enables discrimination between prostate specific antigen from patients with prostate cancer and benign prostatic hyperplasia. Glycobiology. 19 (11), p. 1318. https://doi.org/10.1093/glycob/cwp135

Altered glycosylation of proteins in cancer: what is the potential for new anti-tumour strategies
Brooks, S.A., Carter, T.M., Royle, L., Harvey, D.J., Fry, S., Kinch, C., Dwek, R.A. and Rudd, P.M. 2008. Altered glycosylation of proteins in cancer: what is the potential for new anti-tumour strategies. Anti-Cancer Agents in Medicinal Chemistry. 8 (1), pp. 2-21.

The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors
Van den Steen, P.E., Van Aelst, I., Hvidberg, V., Piccard, H., Fiten, P., Jacobsen, C., Moestrup, S.K., Fry, S., Royle, L., Wormald, M.R., Wallis, R., Rudd, P.M., Dwek, R.A. and Opdenakker, G. 2006. The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors. Journal of Biological Chemistry. 281 (27), pp. 18626-18637.

Cancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3
Fry, S., Van den Steen, P.E., Royle, L., Wormald, M.R., Leathem, A., Opdenakker, G., McDonnell, J.M., Dwek, R.A. and Rudd, P.M. 2006. Cancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3. Biochemistry. 45 (51), pp. 15249-15258. https://doi.org/10.1021/bi061254l

Differential glycosylation of gelatinase B from neutrophils and breast cancer cells
Fry, S., Van den Steen, P.E., Royle, L., Wormald, M.R., Leathem, A., Opdenakker, G., Rudd, P.M. and Dwek, R.A. 2005. Differential glycosylation of gelatinase B from neutrophils and breast cancer cells. Advances in Experimental Medicine and Biology. 564, pp. 103-112. https://doi.org/10.1007/0-387-25515-X_18

Permalink - https://westminsterresearch.westminster.ac.uk/item/8zxz5/lectin-microarray-profiling-of-metastatic-breast-cancers


Share this

Usage statistics

103 total views
0 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.