2DE- based proteomics for the analysis of metastasis associated proteins

Dwek, Miriam and Peiris, Diluka (2012) 2DE- based proteomics for the analysis of metastasis associated proteins. Methods in Molecular Biology, 878. pp. 111-120. ISSN 1064-3745

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Official URL: http://dx.doi.org/10.1007/978-1-61779-854-2_7


Two-dimensional electrophoresis (2-DE) is a high-resolution technique for analysis and comparison of complex protein mixtures. With the advent of recent technical developments, its application has become significant in a wide range of fields. This chapter describes a proteomic approach for the analysis of metastasis-associated proteins using pre-fractionation of glycosylated proteins via lectin (HPA) affinity chromatography prior to separation by 2-DE. Guidelines for the preparation and storage of buffers, experimental conditions and protocols of affinity chromatography, isoelectric focussing, and SDS-PAGE conditions are provided. Critical parameters associated with the different steps of 2-DE are discussed.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Rachel Wheelhouse
Date Deposited: 19 Jul 2012 11:06
Last Modified: 19 Jul 2012 11:06
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/10854

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