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2DE- based proteomics for the analysis of metastasis associated proteins

Dwek, Miriam and Peiris, Diluka (2012) 2DE- based proteomics for the analysis of metastasis associated proteins. Methods in Molecular Biology, 878 . pp. 111-120. ISSN 1064-3745

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Official URL: http://dx.doi.org/10.1007/978-1-61779-854-2_7

Abstract

Two-dimensional electrophoresis (2-DE) is a high-resolution technique for analysis and comparison of complex protein mixtures. With the advent of recent technical developments, its application has become significant in a wide range of fields. This chapter describes a proteomic approach for the analysis of metastasis-associated proteins using pre-fractionation of glycosylated proteins via lectin (HPA) affinity chromatography prior to separation by 2-DE. Guidelines for the preparation and storage of buffers, experimental conditions and protocols of affinity chromatography, isoelectric focussing, and SDS-PAGE conditions are provided. Critical parameters associated with the different steps of 2-DE are discussed.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:10854
Deposited On:19 Jul 2012 12:06
Last Modified:19 Jul 2012 12:06

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