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RGS4 inhibits platelet-activating factor receptor phosphorylation and cellular responses

Richardson, Ricardo M. and Marjoram, Robin J. and Barr, Alastair J. and Snyderman, Ralph (2001) RGS4 inhibits platelet-activating factor receptor phosphorylation and cellular responses. Biochemistry, 40 (12). pp. 3583-3588. ISSN 0006-2960

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Official URL: http://dx.doi.org/10.1021/bi0019242

Abstract

To define the role of regulators of G-protein signaling (RGS) in chemoattractant-mediated responses, RGS4 and the receptors for platelet-activating factor (PAFR), formylated peptides (FR), or interleukin-8 (CXCR1) were stably coexpressed in a rat basophilic leukemia (RBL-2H3) cell line. The data demonstrate that RGS4 inhibited responses by PAFR (i.e., phosphoinositide (PI) hydrolysis, Ca2+ mobilization) but not by FR or CXCR1. An N-terminal 33 amino acid deletion mutant of RGS4 (ΔRGS4), deficient in GAP (GTPase activating protein) activity and plasma membrane localization, had no effect on either PAFR, FR, or CXCR1. RGS4, but not ΔRGS4, also blocked phosphorylation of PAFR by platelet-activating factor (PAF) and, unexpectedly, by phorbol 12-myristate 13-acetate (PMA); it also blocked cross-phosphorylation by formylmethionylleucylphenylalanine (fMLP). A point mutant of RGS4 (N88S), deficient in GAP activity but not membrane localization, partially blocked PAFR phosphorylation but had no effect on PAFR-mediated PI hydrolysis and Ca2+ mobilization. Truncation of the cytoplasmic tail of PAFR (mPAFR) resulted in a loss of its susceptibility to inhibition by RGS4. Taken together, the data indicate that of the receptors studied, RGS4 selectively inhibited responses to PAFR, which preferentially couples to Gq. At the level of expression studied, RGS4 did not inhibit FR or CXCR1 which activates Gi to transduce cellular signals. Since the tail-deleted mutant of PAFR was not affected by RGS4, and RGS4 blocked homologous as well as heterologous phosphorylation of this receptor, it is possible that RGS4 interferes sterically with the cytoplasmic tail of PAFR. Thus, in addition to stimulating the GTPase activity of Gα, RGS4 prevents G protein activation by PAFR and the homologous and heterologous phosphorylation of this receptor.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:10869
Deposited On:20 Jul 2012 14:49
Last Modified:20 Jul 2012 14:49

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