Annexin-A1 protein and its relationship to cortisol in human saliva

Fowkes, Robert C., Moradi-Bidhendi, Niloufar, Branceleone, Vincenzo, Gulrez Zariwala, M. , Brady, Daniel, Jessop, David S., Perretti, Mauro and Renshaw, Derek (2013) Annexin-A1 protein and its relationship to cortisol in human saliva. Psychoneuroendocrinology, 38 (5). pp. 722-727. ISSN 0306-4530


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Salivary cortisol is commonly used as a clinical biomarker of endocrine status and also as a marker of psychosocial stress. Annexin-A1 (AnxA1) is an anti-inflammatory protein whose expression is modulated by glucocorticoids. Our principal objectives were to (i) detect the presence of and (ii) measure AnxA1 protein in whole human saliva and to (iii) investigate whether salivary cortisol and AnxA1 are correlated in healthy humans. A total of 37 healthy participants (male and female) were used in the study. Saliva was collected using salivette tubes. Salivary cortisol and AnxA1 protein were sampled at between 3 and 6 time points over 24 h and measured for cortisol and AnxA1 protein using specific ELISA's. The presence of salivary AnxA1 protein was confirmed by Western blotting. AnxA1 protein is detectable in whole human saliva, as detected by Western blot analysis and ELISA. A diurnal rhythm was evident in both salivary cortisol (P < 0.01) and AnxA1 (P < 0.01) and was defined as a significant difference in time 0 (waking) samples compared to ‘bed’ (2300 h) samples. AnxA1 protein did not exhibit an awakening response (P > 0.05), whereas salivary cortisol was significantly elevated between time 0 and 30 min post waking (P < 0.001). AnxA1 protein correlates positively with salivary cortisol, indicating that cortisol is most likely a regulator of AnxA1 in human saliva.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Rachel Wheelhouse
Date Deposited: 18 Oct 2012 10:53
Last Modified: 12 Jun 2014 11:37

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