Kulikova, Nina, Amaglobeli, Nino, Tsertsvadze, Tamar, Tsagareishvili, Paata, Sereda, Liana, Tevzadze, Mariam, Kardava, Lela, Ghirdaladze, Manana, Gachechiladze, Nino, Lukhumaidze, Mariam and Porakishvili, Nino (2009) Association between seropositivity for cytomegalovirus (CMV) and CD4+ cytotoxic T cells expansions in patients with B-cell Chronic Lymphocyte Leukaemia (B-CLL) and healthy controls. Proceedings of the Georgian Academy of Sciences, 7 (3-4). pp. 41-46. ISSN 1512-2123Full text not available from this repository.
B cell chronic lymphocytic leukaemia (B-CLL) is characterized by the clonal expansion of CD5+CD19+CD23+ B cells. During the course of B-CLL, the expansion of neoplastic clone is accompanied by a disbalance between CD4+/CD8+ T cells and by deficiency of T cell function. We have previously shown an expansion of CD4+ perforin (PF)+ cytotoxic T cells (cytT) with undefined specificity in patients with B-CLL. It has been demonstrated by others that the expansion of CD4+PF+ T cells in control individuals is often associated with chronic viral infections. Taking into consideration that B-CLL patients are immunocompromised, with frequent viral infections, we investigated the role of CD4+PF+ cytotoxic T cells in immune responses to one of the most common chronic viral infections - human cytomegalovirus (hCMV). We studied an association of cytT cell frequencies with the chronic CMV infection in 32 B-CLL patients and 18 age-matched healthy controls. Peripheral blood mononuclear cells (PBMCs) were immunostained with anti-CD4~PerCP monoclonal antibodies (mAb), fixed, permeabilised and immunostained with anti-PF~FITC mAb. Cells were fixed and analyzed by flow cytometry. Serum samples were routinely tested for anti-IgG antibodies to CMV. Here we show that CD4+PF+ T cell expansions appeared to be strongly associated with CMV seropositivity in healthy individuals, and, particularly, in B-CLL patients. The immunocompromised status of the majority of B-CLL patients may facilitate expansion of this unusual population of cytotoxic cells to combat reactivation of a chronic CMV infection.
|Subjects:||University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)|
|Depositing User:||Rachel Wheelhouse|
|Date Deposited:||25 Oct 2012 13:56|
|Last Modified:||11 Jun 2013 11:16|
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