Application of phage display to high throughput antibody generation and characterization

Schofield, Darren J. and Pope, Anthony R. and Clementel, Veronica and Buckell, Jenny and Chapple, Susan D.J. and Clarke, Kay F. and Conquer, Jennie S. and Crofts, Anna M. and Crowther, Sandra R.E. and Dyson, Michael R. and Flack, Gillian and Griffin, Gareth J. and Hooks, Yvette and Howat, William J. and Kolb-Kokocinski, Anja and Kunze, Susan and Martin, Cecile D. and Maslen, Gareth L. and Mitchell, Joanne N. and O'Sullivan, Maureen and Perera, Rajika L. and Roake, Wendy and Shadbolt, S. Paul and Vincent, Karen J. and Warford, Anthony and Wilson, Wendy E. and Xie, Jane and Young, Joyce L. and McCafferty, John (2007) Application of phage display to high throughput antibody generation and characterization. GenomeBiology.com , 8 (11). R254. ISSN 1465-6906

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Official URL: http://dx.doi.org/10.1186/gb-2007-8-11-r254

Abstract

We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Rachel Wheelhouse
Date Deposited: 25 Oct 2012 11:09
Last Modified: 25 Oct 2012 11:09
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/11321

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