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Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)

Deutsch, Eric W. and Ball, Catherine A. and Berman, Jules J. and Bova, G. Steven and Brazma, Alvis and Bumgarner, Roger E. and Campbell, David and Causton, Helen C. and Christiansen, Jeffrey H. and Daian, Fabrice and Dauga, Delphine and Davidson, Duncan R. and Gimenez, Gregory and Goo, Young Ah and Grimmond, Sean and Henrich, Thorsten and Herrmann, Bernhard G and Johnson, Michael H. and Korb, Martin and Mills, Jason C. and Oudes, Asa J. and Parkinson, Helen E. and Pascal, Laura E. and Pollet, Nicolas and Quackenbush, John and Ramialison, Mirana and Ringwald, Martin and Salgado, David and Sansone, Susanna-Assunta and Sherlock, Gavin and Stoeckert, Jr., Christian J. and Swedlow, Jason and Taylor, Ronald C. and Walashek, Laura and Warford, Anthony and Wilkinson, David G. and Zhou, Yi and Zon, Leonard I. and Liu, Alvin Y. and True, Lawrence D. (2008) Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). Nature Biotechnology, 26 (3). pp. 305-312. ISSN 1087-0156

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Official URL: http://dx.doi.org/10.1038/nbt1391

Abstract

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:11322
Deposited On:25 Oct 2012 12:27
Last Modified:25 Oct 2012 12:27

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