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Engagement of different Fc-gamma receptors in phagocytosis of immune complexes containing antibodies to mutated human chorionic gonadotropin β chain (hCG-beta) and native hCG molecule

Chikadze, Nino and Janikashvili, Nona and Gachechiladze, Nino and Lomishvili , Nino and Kvirkvelia, Nino and Delves, Peter J. and Porakishvili, Nino (2009) Engagement of different Fc-gamma receptors in phagocytosis of immune complexes containing antibodies to mutated human chorionic gonadotropin β chain (hCG-beta) and native hCG molecule. Proceedings of the Georgian Academy of Sciences, 7 (1-2). pp. 39-46. ISSN 1512-2123

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Abstract

The aim of this study was to investigate the role of Fcγ receprors (FcγR) in vitro, in phagocytosis of hormone-antibody complexes formed after immunization with the mutant hCGβ(R68E). Native, FITC labeled hCG was added to the sera from mutant hCGβ(R68E) immunized rabbits. The suspensions of phagocytic cells were selectively incubated with monoclonal antibodies to FcγRI, FcγRII, FcγRIII and the complement receptor 3 in order to block their functions and phagocytosis was visualized by flow cytometry. We show that the phagocytosis of immune complexes containing anti-hCGβ(R68E) sera of rabbits and native hCG molecules by monocytes and neutrophils is mediated by FcγRI(CD64) and FcγRIII(CD16) respectively in cooperation with the complement receptor 3 (CR3).

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:11331
Deposited On:25 Oct 2012 15:01
Last Modified:08 Jan 2013 14:57

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