Engagement of different Fc-gamma receptors in phagocytosis of immune complexes containing antibodies to mutated human chorionic gonadotropin ? chain (hCG-beta) and native hCG molecule

Chikadze, Nino and Janikashvili, Nona and Gachechiladze, Nino and Lomishvili , Nino and Kvirkvelia, Nino and Delves, Peter J. and Porakishvili, Nino (2009) Engagement of different Fc-gamma receptors in phagocytosis of immune complexes containing antibodies to mutated human chorionic gonadotropin ? chain (hCG-beta) and native hCG molecule. Proceedings of the Georgian Academy of Sciences, 7 (1-2). pp. 39-46. ISSN 1512-2123

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Abstract

The aim of this study was to investigate the role of Fc? receprors (Fc?R) in vitro, in phagocytosis of hormone-antibody complexes formed after immunization with the mutant hCG?(R68E). Native, FITC labeled hCG was added to the sera from mutant hCG?(R68E) immunized rabbits. The suspensions of phagocytic cells were selectively incubated with monoclonal antibodies to Fc?RI, Fc?RII, Fc?RIII and the complement receptor 3 in order to block their functions and phagocytosis was visualized by flow cytometry. We show that the phagocytosis of immune complexes containing anti-hCG?(R68E) sera of rabbits and native hCG molecules by monocytes and neutrophils is mediated by Fc?RI(CD64) and Fc?RIII(CD16) respectively in cooperation with the complement receptor 3 (CR3).

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Rachel Wheelhouse
Date Deposited: 25 Oct 2012 14:01
Last Modified: 08 Jan 2013 14:57
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/11331

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