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Using electrophoretic deposition to identify protein charge in biological medium

Meng, Decheng and Francis, Lydia and Roy, Ipsita and Boccaccini, Aldo R. (2011) Using electrophoretic deposition to identify protein charge in biological medium. Journal of Applied Electrochemistry, 41 (8). pp. 919-923. ISSN 0021-891X

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Official URL: http://dx.doi.org/10.1007/s10800-011-0317-9

Abstract

Protein adsorption is the first step involved in establishing a suitable integration between a biomaterial and host tissue. It is therefore of highest interest to know the electric charge of proteins present in the relevant medium to be able to predict the behaviour of cells on given surfaces. In this study, electrophoretic deposition (EPD) was used as a simple method to identify the charge of proteins present in biological medium. In the model experiment carried out here, EPD was conducted using a biological medium containing 10% fetal calf serum (FCS) and the charge of the protein was determined by examining the migration of the protein in the EPD cell under a certain applied voltage. In addition, the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:11373
Deposited On:26 Oct 2012 11:25
Last Modified:26 Oct 2012 11:25

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