Thomson, Nicholas and Channon, Kevin and Mokhtar, Noor Azlin and Staniewicz, Lech and Rai, Ranjana and Roy, Ipsita and Sato, Shun and Tsuge, Takeharu and Donald, Athene M. and Summers, David and Sivaniah, Easan (2011) Imaging internal features of whole, unfixed bacteria. Scanning, 33 (2). pp. 59-68. ISSN 0161-0457Full text not available from this repository.
Wet scanning-transmission electron microscopy (STEM) is a technique that allows high-resolution transmission imaging of biological samples in a hydrated state, with minimal sample preparation. However, it has barely been used for the study of bacterial cells. In this study, we present an analysis of the advantages and disadvantages of wet STEM compared with standard transmission electron microscopy (TEM). To investigate the potential applications of wet STEM, we studied the growth of polyhydroxyalkanoate and triacylglycerol carbon storage inclusions. These were easily visible inside cells, even in the early stages of accumulation. Although TEM produces higher resolution images, wet STEM is useful when preservation of the sample is important or when studying the relative sizes of different features, since samples do not need to be sectioned. Furthermore, under carefully selected conditions, it may be possible to maintain cell viability, enabling new types of experiments to be carried out. To our knowledge, internal features of bacterial cells have not been imaged previously by this technique.
|Subjects:||University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)|
|Depositing User:||Rachel Wheelhouse|
|Date Deposited:||26 Oct 2012 10:59|
|Last Modified:||26 Oct 2012 10:59|
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