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IgG_{4} Pf NPNA-1 a human anti-Plasmodium falciparum sporozoite monoclonal antibody cloned from a protected individual inhibits parasite invasion of hepatocytes

Chappel, Jonathan A. and Hollingdale, Michael R. and Kang, Angray S. (2004) IgG_{4} Pf NPNA-1 a human anti-Plasmodium falciparum sporozoite monoclonal antibody cloned from a protected individual inhibits parasite invasion of hepatocytes. Human Antibodies, 13 (3). pp. 91-96. ISSN 1093-2607

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Official URL: http://iospress.metapress.com/openurl.asp?genre=ar...

Abstract

Malaria is one of the world's most devastating diseases, and Plasmodium falciparum (Pf) causes significant mortalities particularly in Sub-Saharan Africa. The rise and spread of multi-drug resistant strains of the parasite has coincided with an era of increased travel to malaria endemic regions. In the absence of an effective vaccine against malaria it may be possible to utilize human monoclonal antibodies against the stage transmitted by mosquito bites (sporozoites) as a prophylactic to prevent infection. We report the characterization of an engineered human IgG_{4} monoclonal antibody against Pf sporozoite cloned from a protected individual recognized the sporozoite surface and inhibited sporozoite invasion of human hepatocytes in vitro. The fully human monoclonal antibody PfNPNA-1 IgG_{4} against (NPNA)_{3} specifically labels Plasmodium falciparum in an IFA. This antibody also inhibits Plasmodium falciparum sporozoite invasion of human hepatocytes HepG2-A16 in a dose dependent manner in an in vitro assay. PfNPNA-1 IgG_{4} is a promising candidate for evaluation for the prevention of malaria.

Item Type:Article
Uncontrolled Keywords:Plasmodium falciparum, sporozoite, human monoclonal antibodies
Research Community:University of Westminster > Life Sciences, School of
ID Code:1545
Deposited On:12 May 2006
Last Modified:11 Aug 2010 15:30

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