In situ hybridisation: Technologies and their application to understanding disease

Warford, A. 2016. In situ hybridisation: Technologies and their application to understanding disease. Progress in Histochemistry and Cytochemistry. 50 (4), pp. 37-48. https://doi.org/10.1016/j.proghi.2015.12.001

TitleIn situ hybridisation: Technologies and their application to understanding disease
AuthorsWarford, A.
Abstract

In situ hybridisation (ISH) is unique amongst molecular analysis methods in providing for the precise microscopic localisation of genes, mRNA and microRNA in metaphase spreads,cell and tissue preparations. The method is well established as a tool to guide appropriate therapeutic intervention in breast, gastric and lung cancer. With the description of ultrasensitive ISH technologies for low copy mRNA demonstration and the relative ease by which microRNA can be visualised, the applications for research and diagnostic purposes is set to increase dramatically. In this review ISH is considered with emphasis on recent technological developments and surveyed for present and future applications in the context of the demonstration of genes, mRNA and microRNA in health and disease.

KeywordsIn situ hybridisation,DNA amplification, mRNA expression, microRNA localisationa
JournalProgress in Histochemistry and Cytochemistry
Journal citation50 (4), pp. 37-48
ISSN0079-6336
Year2016
PublisherElsevier
Digital Object Identifier (DOI)https://doi.org/10.1016/j.proghi.2015.12.001
Publication dates
PublishedJan 2016
Published25 Dec 2016

Related outputs

Digestive system
Warford, A. and Madgwick, A. 2015. Digestive system. in: Orchard, G. and Nation, B. (ed.) Cell Structure and Function Oxford UK Oxford University Press. pp. 220-245

Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers
Howat, W.J., Lewis, A., Jones, P., Kampf, C., Pontén, F., F, van der Loos, C.M., Gray, N., Womack, C. and Warford, A. 2014. Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers. Methods. 70 (1), pp. 34-38. https://doi.org/10.1016/j.ymeth.2014.01.018

Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems.
Warford, A., Akbar, H. and Riberio, D. 2014. Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems. Methods. 70 (1), p. 28–33. https://doi.org/10.1016/j.ymeth.2014.03.001

Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G
Eagle, R.A., Flack, G., Warford, A., Martínez-Borra, J., Jafferji, I., Traherne, J.A., Ohashi, M., Boyle, L.H., Barrow, A.D., Caillat-Zucman, S., Young, N.T. and Trowsdale, J. 2009. Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G. PLoS ONE. 4 (2) e4503. https://doi.org/10.1371/journal.pone.0004503

Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)
Deutsch, E.W., Ball, C.A., Berman, J.J., Bova, G.S., Brazma, A., Bumgarner, R.E., Campbell, D., Causton, H.C., Christiansen, J.H., Daian, F., Dauga, D., Davidson, D.R., Gimenez, G., Goo, Y.A., Grimmond, S., Henrich, T., Herrmann, B.G., Johnson, M.H., Korb, M., Mills, J.C., Oudes, A.J., Parkinson, H.E., Pascal, L.E., Pollet, N., Quackenbush, J., Ramialison, M., Ringwald, M., Salgado, D., Sansone, S.A., Sherlock, G., Stoeckert Jr, C.J., Swedlow, J., Taylor, R.C., Walashek, L., Warford, A., Wilkinson, D., Zhou, Y., Zon, L.I., Liu, A.Y., True, L.D. and Stoeckert Jr, Christian J. 2008. Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). Nature Biotechnology. 26 (3), pp. 305-312. https://doi.org/10.1038/nbt1391

Targeting therapeutics: closing the gap between bench and bedside. A focus on tissue microarrays
Warford, A. 2007. Targeting therapeutics: closing the gap between bench and bedside. A focus on tissue microarrays. Expert Review of Molecular Diagnostics. 7 (2), pp. 103-105. https://doi.org/10.1586/14737159.7.2.103

Image capture, analysis and quantification
Wu, J., Warford, A. and Tannahill, D. 2007. Image capture, analysis and quantification. in: Renshaw, S. (ed.) Immunohistochemistry Scion Publishing Ltd. pp. 175-204

Application of phage display to high throughput antibody generation and characterization
Schofield, D.J., Pope, A.R., Clementel, V., Buckell, J., Chapple, S.D.J., Clarke, K.F., Conquer, J.S., Crofts, A.M., Crowther, S.R.E., Dyson, M.R., Flack, G., Griffin, G.J., Hooks, Y., Howat, W.J., Kolb-Kokocinski, A., Kunze, S., Martin, C.D., Maslen, G.L., Mitchell, J.N., O'Sullivan, M., Perera, R.L., Roake, W., Shadbolt, S.P., Vincent, K.J., Warford, A., Wilson, W.E., Xie, J., Young, J.L. and McCafferty, J. 2007. Application of phage display to high throughput antibody generation and characterization. GenomeBiology.com. 8 (11), p. R254. https://doi.org/10.1186/gb-2007-8-11-r254

Assessing the potential of immunohistochemistry for systematic gene expression profiling
Warford, A., Flack, G., Conquer, J.S., Zola, H. and McCafferty, J. 2007. Assessing the potential of immunohistochemistry for systematic gene expression profiling. Journal of Immunological Methods. 318 (1-2), pp. 125-137. https://doi.org/10.1016/j.jim.2006.10.011

Permalink - https://westminsterresearch.westminster.ac.uk/item/9v545/in-situ-hybridisation-technologies-and-their-application-to-understanding-disease


Share this

Usage statistics

98 total views
0 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.