Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Lange, S., Stratton, D. and Inal, J. (2013) Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells. Biochemical and Biophysical Research Communications, 430 (2). pp. 470-475. ISSN 0006-291X

Full text not available from this repository.
Official URL: https://dx.doi.org/10.1016/j.bbrc.2012.12.012

Abstract

Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6 V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.

Item Type: Article
Uncontrolled Keywords: Extremely low-frequency magnetic field; Stimulated microvesicle; Calcium homeostasis; Plasma membrane;
Subjects: University of Westminster > Science and Technology
SWORD Depositor: repository@westminster.ac.uk
Depositing User: repository@westminster.ac.uk
Date Deposited: 11 Feb 2016 15:07
Last Modified: 02 Aug 2016 10:31
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/16482

Actions (login required)

Edit Item (Repository staff only) Edit Item (Repository staff only)