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Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

Dwek, Miriam and Ross, Heidi and Streets, Andrew and Brooks, Susan and Adam, Elizabeth and Titcomb, Ann and Woodside, Jayne and Schumacher, Udo and Leathem, Anthony (2001) Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. International Journal of Cancer, 95 (2). pp. 79-85. ISSN 0020-7136

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Official URL: http://dx.doi.org/10.1002/1097-0215(20010320)95:2<...

Abstract

In a study for the identification of genomic alterations in pancreatic cancer, representational difference analysis was used and led to the isolation of 2 distinct fragments, deleted on the Y chromosome in the xenografted tumor DNA of a male patient with an adenocarcinoma of the pancreas. Loss of Y chromosomal material was further studied in 11 pancreatic cancer cell lines of male origin, using PCR amplification of 5 sequence tagged sites (STSs) distributed along the Y chromosome; 8/11 cell lines exhibited a complete loss of the Y chromosome and 3 had deletions. To examine the status of the Y chromosome in situ, interphase FISH analysis was performed on paraffin sections from pancreatic carcinoma (n=7) and chronic pancreatitis (n=7) tissues, and the loss of Y-chromosomal STS-markers was studied in 6 xenograft tumors obtained from male pancreatic cancer patients. This analysis revealed that a loss of the Y chromosome occurs in vivo in primary pancreatic tumor cells, whereas the Y chromosome was intact in chronic pancreatitis. Our data suggest that loss of Y is a frequent event occurring in male pancreatic tumors. Although there is no evidence for a functional implication of Y chromosome loss, it effectively differentiates between a malignant and a benign condition as e.g. chronic pancreatitis. Thus, this genetic alteration may be of diagnostic use.

Item Type:Article
Additional Information:Online ISSN 1097-0215
Research Community:University of Westminster > Life Sciences, School of
ID Code:243
Deposited On:30 Aug 2005
Last Modified:15 Dec 2009 10:47

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