Dwek, Miriam and Ross, Heidi and Streets, Andrew and Brooks, Susan and Adam, Elizabeth and Titcomb, Ann and Woodside, Jayne and Schumacher, Udo and Leathem, Anthony (2001) Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. International Journal of Cancer, 95 (2). pp. 79-85. ISSN 0020-7136
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Official URL: http://dx.doi.org/10.1002/1097-0215(20010320)95:2<...
In a study for the identification of genomic alterations in pancreatic cancer, representational difference analysis was used and led to the isolation of 2 distinct fragments, deleted on the Y chromosome in the xenografted tumor DNA of a male patient with an adenocarcinoma of the pancreas. Loss of Y chromosomal material was further studied in 11 pancreatic cancer cell lines of male origin, using PCR amplification of 5 sequence tagged sites (STSs) distributed along the Y chromosome; 8/11 cell lines exhibited a complete loss of the Y chromosome and 3 had deletions. To examine the status of the Y chromosome in situ, interphase FISH analysis was performed on paraffin sections from pancreatic carcinoma (n=7) and chronic pancreatitis (n=7) tissues, and the loss of Y-chromosomal STS-markers was studied in 6 xenograft tumors obtained from male pancreatic cancer patients. This analysis revealed that a loss of the Y chromosome occurs in vivo in primary pancreatic tumor cells, whereas the Y chromosome was intact in chronic pancreatitis. Our data suggest that loss of Y is a frequent event occurring in male pancreatic tumors. Although there is no evidence for a functional implication of Y chromosome loss, it effectively differentiates between a malignant and a benign condition as e.g. chronic pancreatitis. Thus, this genetic alteration may be of diagnostic use.
|Additional Information:||Online ISSN 1097-0215|
|Research Community:||University of Westminster > Life Sciences, School of|
|Deposited On:||30 Aug 2005|
|Last Modified:||15 Dec 2009 10:47|
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