Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

Dwek, Miriam, Ross, Heidi, Streets, Andrew, Brooks, Susan, Adam, Elizabeth, Titcomb, Ann, Woodside, Jayne, Schumacher, Udo and Leathem, Anthony (2001) Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. International Journal of Cancer, 95 (2). pp. 79-85. ISSN 0020-7136

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Official URL: http://dx.doi.org/10.1002/1097-0215(20010320)95:2<...

Abstract

In a study for the identification of genomic alterations in pancreatic cancer, representational difference analysis was used and led to the isolation of 2 distinct fragments, deleted on the Y chromosome in the xenografted tumor DNA of a male patient with an adenocarcinoma of the pancreas. Loss of Y chromosomal material was further studied in 11 pancreatic cancer cell lines of male origin, using PCR amplification of 5 sequence tagged sites (STSs) distributed along the Y chromosome; 8/11 cell lines exhibited a complete loss of the Y chromosome and 3 had deletions. To examine the status of the Y chromosome in situ, interphase FISH analysis was performed on paraffin sections from pancreatic carcinoma (n=7) and chronic pancreatitis (n=7) tissues, and the loss of Y-chromosomal STS-markers was studied in 6 xenograft tumors obtained from male pancreatic cancer patients. This analysis revealed that a loss of the Y chromosome occurs in vivo in primary pancreatic tumor cells, whereas the Y chromosome was intact in chronic pancreatitis. Our data suggest that loss of Y is a frequent event occurring in male pancreatic tumors. Although there is no evidence for a functional implication of Y chromosome loss, it effectively differentiates between a malignant and a benign condition as e.g. chronic pancreatitis. Thus, this genetic alteration may be of diagnostic use.

Item Type: Article
Additional Information: Online ISSN 1097-0215
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Users 4 not found.
Date Deposited: 30 Aug 2005
Last Modified: 15 Dec 2009 10:47
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/243

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