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Lipolytic enzyme production in batch and fed-batch cultures of Ophiostoma piceae and Fusarium oxysporum

Tamerler, Candan and Keshavarz, Tajalli (2000) Lipolytic enzyme production in batch and fed-batch cultures of Ophiostoma piceae and Fusarium oxysporum. Journal of Chemical Technology and Biotechnology, 75 (9). pp. 785-790. ISSN 0268-2575

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Official URL: http://dx.doi.org/10.1002/1097-4660(200009)75:9<78...

Abstract

Lipase and esterase production by Ophiostoma piceae and Fusarium oxysporum were enhanced and extended by developing a fed-batch process in stirred tank reactors. Fed-batch strategy improved lipolytic enzyme production from Ophiostoma piceae in both 2 and 20 dm3 stirred tank reactors. However, fed-batch fermentation of Fusarium oxysporum in the 2 dm3 reactor was more effective than both batch and fed-batch fermentations in the 20 dm3 reactor. When a medium composed of only carbon and nitrogen source was intermittently fed to the cultures, the maximum specific lipase activity was improved by more than 80% and 35% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The maximum specific esterase activity was improved by 20% and 15% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The duration of production for both fungi extended from 144 to 216 h compared with a batch culture under the same condition.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:2660
Deposited On:10 Nov 2006
Last Modified:11 Dec 2009 11:56

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