Rughooputh, Sanjiv and Parmar, K. and Greenwell, Pamela (2004) Detection of human papillomavirus from liquid-based cytology specimens by in-house PCR: a pilot study. British Journal of Biomedical Science, 61 (1). pp. 22-25. ISSN 0967-4845
Official URL: http://www.bjbs-online.org/article.asp?id=131
The Papanicolaou smear remains the most common method for the detection of precancerous changes in cervical cytology. However, the introduction of a liquidbased cytology (LBC) technique expands the possibility of cervical intraepithelial neoplasia (CIN) diagnosis, and permits detection of precancerous changes and human papillomavirus (HPV) simultaneously. In the pilot study reported here, using an in-house polymerase chain reaction (PCR) method, high-grade HPV was detected in 32% of a cohort of 38 patients. This conventional PCR method could be developed for use on a real-time PCR platform or in a microtitre-well format and subsequently automated.
|Uncontrolled Keywords:||Cervical intraepithelial neoplasia, Cervical neoplasia. Human papillomavirus, Liquid-based cytology, Polymerase chain reaction|
|Research Community:||University of Westminster > Life Sciences, School of|
|Deposited On:||01 Dec 2005|
|Last Modified:||11 Aug 2010 15:29|
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