Detection of human papillomavirus from liquid-based cytology specimens by in-house PCR: a pilot study

Rughooputh, Sanjiv and Parmar, K. and Greenwell, Pamela (2004) Detection of human papillomavirus from liquid-based cytology specimens by in-house PCR: a pilot study. British Journal of Biomedical Science, 61 (1). pp. 22-25. ISSN 0967-4845

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Official URL: http://www.bjbs-online.org/article.asp?id=131

Abstract

The Papanicolaou smear remains the most common method for the detection of precancerous changes in cervical cytology. However, the introduction of a liquidbased cytology (LBC) technique expands the possibility of cervical intraepithelial neoplasia (CIN) diagnosis, and permits detection of precancerous changes and human papillomavirus (HPV) simultaneously. In the pilot study reported here, using an in-house polymerase chain reaction (PCR) method, high-grade HPV was detected in 32% of a cohort of 38 patients. This conventional PCR method could be developed for use on a real-time PCR platform or in a microtitre-well format and subsequently automated.

Item Type: Article
Uncontrolled Keywords: Cervical intraepithelial neoplasia, Cervical neoplasia. Human papillomavirus, Liquid-based cytology, Polymerase chain reaction
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Users 4 not found.
Date Deposited: 01 Dec 2005
Last Modified: 11 Aug 2010 14:29
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/277

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