Use of bioinformatics and PCR in the search for ABC transporter homology among various bacteria

Jauhangeer, B.R. and Wren, M.W.D. and MacDonald, R.A.C. and Perry, David and Greenwell, Pamela (2004) Use of bioinformatics and PCR in the search for ABC transporter homology among various bacteria. British Journal of Biomedical Science, 61 (4). pp. 182-185. ISSN 0967-4845

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Abstract

Bioinformatics databases and search tools are utilised to produce polymerase chain reaction (PCR) primers for the amplification of an ABC transporter gene from the clinically important anaerobe Finegoldia magna. On sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of Enterococcus faecalis. Little sequence data is available for F. magna and the newly isolated DNA could be a useful tool in the identification of this organism in clinical specimens.

Item Type: Article
Uncontrolled Keywords: ABC transporters, Computational biology, Finegoldia magna
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Users 4 not found.
Date Deposited: 01 Dec 2005
Last Modified: 11 Aug 2010 14:29
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/280

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