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Intracellular expression of the truncated extracellular domain of c-erbB-3/HER3

Srinivasan, Radhika and Leverton, Kate and Sheldon, Helen and Hurst, Helen and Sarraf, Catherine and Gullick, William (2001) Intracellular expression of the truncated extracellular domain of c-erbB-3/HER3. Cellular Signalling, 13 (5). pp. 321-330. ISSN 0898-6568

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Official URL: http://dx.doi.org/10.1016/S0898-6568(01)00155-3

Abstract

The ERBB3 gene is expressed as a 6.2- and a 1.4-kb transcript. The former encodes the full-length transmembrane protein and the latter a truncated extracellular fragment consisting of 140 amino acids of the c-erbB-3 protein followed by 43 unique residues. We have examined the expression of the two ERBB3 transcripts by Northern blotting in cancer cell lines and normal human fetal and adult tissues. We expressed the truncated receptor fragment and showed that it was glycosylated, probably with a single N-linked complex sugar chain, and that the protein was a 58-kDa disulphide-linked dimer. We were able to crosslink iodinated neuregulin (NRG)-1β to the full-length solubilised receptor but not to the truncated dimeric protein. Using Western blot analysis, the truncated protein was shown to be present in cell lysates and, using immunoelectron microscopy, in vesicular structures within cells and associated with the plasma cell membrane

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:338
Deposited On:01 Dec 2005
Last Modified:23 Dec 2009 15:31

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