Radman, Romeo and Bucke, Christopher and Keshavarz, Tajalli (2004) Elicitor effects on Penicillium chrysogenum morphology in submerged cultures. Biotechnology and Applied Biochemistry, 40 (3). pp. 229-233. ISSN 0885-4513
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Official URL: http://dx.doi.org/10.1042/BA20040062
Changes in morphology and sporulation were investigated in liquid cultures of Penicillium chrysogenum P2 supplemented with carbohydrate oligosaccharides. Sodium alginate and locust-bean (Ceratonia siliqua) gum-derived oligosaccharides were used as elicitors. Spore germination was inhibited by the addition of OG (oligoguluronate) elicitor (30% inhibition when compared with control). Addition of any of the elicitors to stirred-tank cultures increased hyphal-tip numbers, clump area and spore counts. MO (mannan oligosaccharide) had the greatest effect on the parameters studied, followed by OM (oligomannuronate) and OG. Hyphal-tip numbers increased by 19, 29 and 47% with OG, OM and MO respectively. Average clump area in the presence of OG, OM and MO increased by 23, 32 and 59% respectively. A notable increase in spore numbers was observed in all the supplemented stirred-tank reactor cultures, with MO showing the highest enhancement.
|Additional Information:||Online ISSN 1470-8744|
|Uncontrolled Keywords:||Elicitor, liquid culture, morphology, oligosaccharide, Penicillium chrysogenum|
|Research Community:||University of Westminster > Life Sciences, School of|
|Deposited On:||01 Dec 2005|
|Last Modified:||11 Dec 2009 11:50|
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