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Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries

Kang, Angray S. and Jones, Teresa M. and Burton, Dennis R. (1991) Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries. Proceedings of the National Academy of Sciences, 88 (24). pp. 11120-11123. ISSN 0027-8424

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Official URL: http://www.pnas.org/cgi/reprint/88/24/11120.pdf

Abstract

A number of experiments on the shuffling of heavy and light chains from antibodies of defined specificity for the transition-state analogue hapten nitrophenyl phosphonamidate are described. The experiments report on the promiscuity of heavy and light chains in binding antigen and the feasibility of antibody redesign by this shuffing process. The concepts of incestuous and extraclonal promiscuous association are described. Shuffling opens the possibility of generating panels of antibodies with related specificity but of distinct idiotypic composition that may have significance in the use of human monoclonal antibodies in therapy.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:4531
Deposited On:02 Jan 2008
Last Modified:23 Dec 2009 14:27

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