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Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces

Kang, Angray S. and Barbas III, Carlos F. and Janda, Kim J. and Benkovic, Stephen J. and Lerner, Richard A. (1991) Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proceedings of the National Academy of Sciences, 88 (10). pp. 4363-4366. ISSN 0027-8424

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Official URL: http://www.pnas.org/cgi/reprint/88/10/4363.pdf

Abstract

We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of ifiamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:4534
Deposited On:02 Jan 2008
Last Modified:23 Dec 2009 14:23

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