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Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

Huse, W.D. and Sastry, L. and Iverson, S.A. and Kang, Angray S. and Alting-Mees, M. and Burton, Dennis R. and Benkovic, Stephen J. and Lerner, Richard A. (1989) Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science, 246 (4935). pp. 1275-1281. ISSN 0036-8075

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Official URL: http://dx.doi.org/10.1126/science.2531466

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:4547
Deposited On:07 Jan 2008
Last Modified:23 Dec 2009 14:24

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