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Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley

Morgan, Michael R.A. and Kang, Angray S. and Chan, Henry W-S. (1986) Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley. Journal of the Science of Food and Agriculture, 37 (9). pp. 873-880. ISSN 0022-5142

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Official URL: http://dx.doi.org/10.1002/jsfa.2740370911

Abstract

An enzyme-linked immunosorbent assay for the detection of 3-acetyldeoxynivalenol has been set up with an antiserum preparation raised against a bovine serum albumin - 3-acetyldeoxynivalenol hemiglutarate conjugate. The assay is an indirect, double antibody immunoassay performed on microtitration plates, and is sensitive (with a limit of detection of 1 pg per well) and specific. The assay has been validated for application to rice with a sensitivity of 1 ng g-1. Sample extraction is a simple solubilisation procedure. The immunoassay described has a number of advantages over conventional methods of analysis.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:4557
Deposited On:07 Jan 2008
Last Modified:23 Dec 2009 14:41

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