Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay

Morgan, Michael R.A. and Kang, Angray S. and Chan, Henry W-S. (1986) Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay. Journal of the Science of Food and Agriculture, 37 (9). pp. 908-914. ISSN 0022-5142

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Official URL: http://dx.doi.org/10.1002/jsfa.2740370913

Abstract

High specificity, high titre antisera to aflatoxins B1 and G1 have been produced by immunisation of rabbits with a bovine serum albumin-aflatoxin B1 conjugate. The antiserum has been used to set up an indirect, double antibody microtitration plate enzyme-linked immunosorbent assay with a limit of detection of 0.1 pg toxin per well. The assay has been validated for application to peanut butter, and demonstrates many advantages over conventional methodology, including the requirement for minimal sample preparation before assay, its technical simplicity, and its potential high sample through-put.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Miss Nina Watts
Date Deposited: 07 Jan 2008
Last Modified: 23 Dec 2009 14:40
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/4558

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