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Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins

Afrough, Babak and Dwek, Miriam and Greenwell, Pamela (2007) Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins. BioTechniques, 43 (4). pp. 458-462. ISSN 0736-6205

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Official URL: http://www.biotechniques.com/default.asp?page=arti...

Abstract

Systematic optimization of a lectin-based enzyme-linked immunosorbent assay (ELISA) procedure using a panel of 21 biotinylated plant lectins and a glycoprotein with defined glycosylation (i) identified blocking as a limiting step in solid phase sugar binding analysis and (ii) found Synblock to be a better alternative to bovine serum albumin (BSA) as blocking agent.

Item Type:Article
Uncontrolled Keywords:Evaluation Studies, Research Support, Non-U.S. Gov't
Research Community:University of Westminster > Life Sciences, School of
ID Code:4615
Deposited On:15 Jan 2008
Last Modified:15 Dec 2009 10:37

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