Afrough, Babak and Dwek, Miriam and Greenwell, Pamela (2007) Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins. BioTechniques, 43 (4). pp. 458-462. ISSN 0736-6205
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Official URL: http://www.biotechniques.com/default.asp?page=arti...
Systematic optimization of a lectin-based enzyme-linked immunosorbent assay (ELISA) procedure using a panel of 21 biotinylated plant lectins and a glycoprotein with defined glycosylation (i) identified blocking as a limiting step in solid phase sugar binding analysis and (ii) found Synblock to be a better alternative to bovine serum albumin (BSA) as blocking agent.
|Uncontrolled Keywords:||Evaluation Studies, Research Support, Non-U.S. Gov't|
|Research Community:||University of Westminster > Life Sciences, School of|
|Deposited On:||15 Jan 2008|
|Last Modified:||15 Dec 2009 10:37|
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