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Disruption of methylarginine metabolism impairs vascular homeostasis

Leiper, James M. and Nandi, Manasi and Torondel, Belen and Murray-Rust, Judith and Malaki, Mohammed and O'Hara, Bernard and Rossiter, Sharon and Anthony, Shelagh and Madhani, Melanie and Selwood, David L. and Smith, Caroline L. and Wojciak-Stothard, Beata and Rudiger, Alain and Stidwill, Ray and McDonald, Neil Q. and Vallance, Patrick (2007) Disruption of methylarginine metabolism impairs vascular homeostasis. Nature Medicine, 13 (2). pp. 198-203. ISSN 1078-8956

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Official URL: http://dx.doi.org/10.1038/nm1543

Abstract

Asymmetric dimethylarginine (ADMA) and monomethyl arginine (L-NMMA) are endogenously produced amino acids that inhibit all three isoforms of nitric oxide synthase (NOS)1. ADMA accumulates in various disease states, including renal failure, diabetes and pulmonary hypertension, and its concentration in plasma is strongly predictive of premature cardiovascular disease and death2, 3, 4. Both L-NMMA and ADMA are eliminated largely through active metabolism by dimethylarginine dimethylaminohydrolase (DDAH)5 and thus DDAH dysfunction may be a crucial unifying feature of increased cardiovascular risk. However, despite considerable interest in this pathway and in the role of ADMA as a cardiovascular risk factor, there is little evidence to support a causal role of ADMA in pathophysiology. Here we reveal the structure of human DDAH-1 and probe the function of DDAH-1 both by deleting the DDAH1 gene in mice and by using DDAH-specific inhibitors which, as we demonstrate by crystallography, bind to the active site of human DDAH-1. We show that loss of DDAH-1 activity leads to accumulation of ADMA and reduction in NO signaling. This in turn causes vascular pathophysiology, including endothelial dysfunction, increased systemic vascular resistance and elevated systemic and pulmonary blood pressure. Our results also suggest that DDAH inhibition could be harnessed therapeutically to reduce the vascular collapse associated with sepsis.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:4670
Deposited On:18 Mar 2008
Last Modified:11 Apr 2008 13:33

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