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The three-dimensional structure of codakine and related marine C-type lectins

Gourdine, Jean-Philippe and Markiv, Anatoliy and Smith-Ravin, Juliette (2007) The three-dimensional structure of codakine and related marine C-type lectins. Fish & Shellfish Immunology, 23 (4). pp. 831-839. ISSN 1050-4648

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Official URL: http://dx.doi.org/10.1016/j.fsi.2007.03.009

Abstract

Codakine is a new Ca2+-dependent mannose-binding C-type lectin (MBL) isolated from the gill tissue of the tropical clam, Codakia orbicularis. Bioinformatic analyses with the BLAST program have revealed similarities with marine lectins involved in immunity whose three-dimensional (3D) structures were unknown up until recently. In this article, we present bioinformatic analyses of marine lectins that are homologous to codakine, in particular lectins from the sea worm Laxus oneistus, named mermaid. These lectins are involved in the symbiotic association with sulphur-oxidizing bacteria which are closely related to the C. orbicularis gill symbiont. Using homology modelling, folding that is characteristic of C-type lectins was observed in all the marine Ca2+-dependent lectins studied, with conservation of random coiled structures of the carbohydrate recognition domain (CRD) and Ca2+-binding sites. Like codakine, the marine lectins analysed contain a signal peptide commonly found in secreted and transmembrane proteins. The majority of the predictive 3D models established from the lectins exhibit a common feature, namely the involvement in invertebrate and vertebrate immunity (dendritic cell receptor, macrophage receptor, etc.). These bioinformatic analyses and the literature data support the hypothesis that codakine, like the L. oneistus mermaids, is probably involved in the cellular mediation of symbiosis and defence against pathogenic microorganisms.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:6024
Deposited On:18 Feb 2009 15:01
Last Modified:18 Oct 2012 11:40

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