Multi-functional P(3HB) microsphere/45S5 Bioglass((R))-based composite scaffolds for bone tissue engineering

Francis, Lydia, Meng, Decheng, Knowles, Jonathan C., Roy, Ipsita and Boccaccini, Aldo R. (2010) Multi-functional P(3HB) microsphere/45S5 Bioglass((R))-based composite scaffolds for bone tissue engineering. Acta Biomaterialia, 6 (7). pp. 2773-2786. ISSN 1742-7061

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Novel multi-functional P(3HB) microsphere/45S5 Bioglass((R))-based composite scaffolds exhibiting potential for drug delivery were developed for bone tissue engineering. 45S5 Bioglass((R))-based glass-ceramic scaffolds of high interconnected porosity produced using the foam-replication technique were coated with biodegradable microspheres (size < 2 mum) made from poly(3-hydroxybutyrate), P(3HB), produced using Bacillus cereus SPV. A solid-oil-in-water emulsion solvent extraction /evaporation technique was used to produce these P(3HB) microspheres. A simple slurry-dipping method, using a 1 wt % suspension of P(3HB) microspheres in water, dispersed by an ultrasonic bath, was used to coat the scaffold, producing a uniform microsphere-coating throughout the 3D scaffold structure. Compressive strength tests confirmed that the microsphere-coating slightly enhanced the scaffold mechanical strength. It was also confirmed that the microsphere-coating did not inhibit the bioactivity of the scaffold when immersed in simulated body fluid (SBF) for up to 4 weeks. The hydroxyapatite (HA) growth rate on P(3HB) microsphere coated 45S5 Bioglass((R)) composite scaffolds was very similar to that on the uncoated control sample, qualitatively indicating similar bioactivity. However, the surface topography of the HA surface layer was affected as shown by results obtained from white light interferometry. The roughness of the surface was much higher for the P(3HB) microsphere-coated scaffolds than for the uncoated samples, after seven days in SBF. This feature would facilitate cell attachment and proliferation. Finally, gentamycin was successfully encapsulated into the P(3HB) microspheres to demonstrate the drug delivery capability of the scaffolds. Gentamycin release kinetics was determined using liquid chromatography-mass spectrometry (LC-MS). The release of the drug from the coated composite scaffolds was slow and controlled when compared to the observed fast and relatively uncontrolled drug release from the bone scaffold (without microsphere coating). Thus, this unique multifunctional bioactive composite scaffold has the potential to enhance cell attachment and to provide controlled delivery of relevant drugs for bone tissue engineering.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Miss Nina Watts
Date Deposited: 19 Jan 2010 12:58
Last Modified: 26 May 2010 09:32

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