Study of the effects of oligosaccharides in liquid cultures of penicillium chrysogenum

Radman, R. 2002. Study of the effects of oligosaccharides in liquid cultures of penicillium chrysogenum. PhD thesis University of Westminster School of Life Sciences https://doi.org/10.34737/93y1q

TitleStudy of the effects of oligosaccharides in liquid cultures of penicillium chrysogenum
TypePhD thesis
AuthorsRadman, R.
Abstract

Oligosaccharides and polysaccharides have different effects on the morphology and production of secondary metabolites by Penicillium chrysogenum P2 (ATCC 48271). Addition Of

oligosaccharides, derived from sodium alginate and locust bean gum, to submerged cultures of P. chrysogenum P2, at milligram per litre concentration (150 mgL-1), increased secondary metabolite levels and spore production, caused changes in morphology and gerRiination of spores, and affected the production of Reactive Oxygen Species. The source of the oligosaccharides controlled their effects on the cultures.

Oligosaccharides when added to submerged cultures of P. chrysogenum P2 increased both penicillin G and extracellular levels of 6-aminopenicillanic acid concentrations. The oligosaccharides had no significant effects on biomass levels. Locust bean gum-derived oligosaccharides (mannan oligosaccharides, DP 5-8), showed the highest levels of enhancement in both penicillin G and 6-aminopenicillanic acid concentrations. Sodium alginate-derived oligosaccharides, (oligoguluronate, DP 7 and oligomannuronate, DP 7), also induced elicitation of penicillin G and 6-aminopenicillanic acid. Oligomannuronate was shown to be more effective than oligoguluronate. In P. chrysogenum P2 cultures mannan, oligomannuronate and oligoguluronate oligosaccharides enhanced yields of penicillin G by 101%, 78% and 59%, respectively. Addition of mannan, oligomannuronate and oligoguluronate oligosaccharides enhanced the levels of 6- aminopenicillanic acid by 39%, 26% and 19%, respectively.

The addition of oligosaccharides and polysaccharides to spores of P. chrysogenum P2 in liquid medium had varying (inhibitory or stimulatory) effects on germination, germ-tube and clump development.

The addition of oligosaccharides to submerged cultures of P. chrysogenum P2 showed effects on clump size and hyphal tip numbers. Mannan oligosaccharides had the greatest effect on morphology followed by oligomannuronate and oligoguluronate oligosaccharides.

Oligosaccharides also speeded-up the sporulation and increased the concentration of spores of P. chrysogenum P2 in liquid cultures. Mannan oligosaccharides had the greatest effect followed by oligomannuronate and oligoguluronate oligosaccharides.

8-aminonaphthalene-1,3,6-trisulphonate-tagged oligosaccharide studies showed that the oligosaccharides pass through the cell wall of P. chrysogenum P2 suggesting a possible mechanism through modulation of gene function. The elicitation pattern was shown to be similar to untagged oligosaccharides.

Oligosaccharides and polysaccharides were shown to inhibit production of Reactive Oxygen Species in P. chrysogenum P2. The highest level of inhibition was elicited by mannan followed by oligornannuronate and oligoguluronate oligosaccharides, and then locust bean gum and alginate. The results of the study showed the potential of oligosaccharides as elicitors of secondary metabolites in P. chrysogenum P2 as a filamentous fungus model. Understanding the elicitation mechanism could provide routes for ftirther exploitation of the potential of filamentous fungi in production of commercial products.

Year2002
File
PublisherUniversity of Westminster
Publication dates
Published2002
Digital Object Identifier (DOI)https://doi.org/10.34737/93y1q

Related outputs

Effect of mannan oligosaccharide elicitor and ferulic acid on enhancement of laccases production in liquid cultures of basidiomycetes
Vanhulle, S., Radman, R., Parra, R., Cui, T., Bols, C.M., Tron, T., Sannia, G. and Keshavarz, T. 2007. Effect of mannan oligosaccharide elicitor and ferulic acid on enhancement of laccases production in liquid cultures of basidiomycetes. Enzyme and Microbial Technology. 40 (7), pp. 1712-1718. https://doi.org/10.1016/j.enzmictec.2006.10.002

Novel application of oligosaccharides as elicitors for the enhancement of bacitracin A production in cultures of Bacillus licheniformis
Murphy, T., Parra, R., Radman, R., Roy, I., Harrop, A., Dixon, K. and Keshavarz, T. 2007. Novel application of oligosaccharides as elicitors for the enhancement of bacitracin A production in cultures of Bacillus licheniformis. Enzyme and Microbial Technology. 40 (6), pp. 1518-1523. https://doi.org/10.1016/j.enzmictec.2006.10.030

Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems
Radman, R., Bland, E.J., Sangworachat, N., Bucke, C. and Keshavarz, T. 2006. Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems. Biotechnology and Applied Biochemistry. 44 (3), pp. 129-133. https://doi.org/10.1042/BA20050217

Biological activity of industrial wood and bark waste from Pinus nigra
Curras Lino, M., Radman, R., Bertaud, F., Nisula, L., Lenon, G., Holbom, B., Locke, I.C., Thompson, S. and Keshavarz, T. 2006. Biological activity of industrial wood and bark waste from Pinus nigra. in: Actual problems of creation of new medicinal preparations of natural origin: the 10th international congress PHYTOPHARM 2006, St.-Petersburg, June 27-30, 2006: proceedings of congress St Petersburg, Russia PHYTOPHARM. pp. 401-403

Towards unravelling the elicitation mechanism in cultures of Penicillium chrysogenin: Chrysogenin elicitation
Nair, R.R., Radman, R., Roy, I., Bucke, C. and Keshavarz, T. 2005. Towards unravelling the elicitation mechanism in cultures of Penicillium chrysogenin: Chrysogenin elicitation. in: Pierucci, S. (ed.) 7th Italian Conference on Chemical and Process Engineering: ICheaP 7, May 15-16, 2005, Giardini Di Naxos, Italy Italy Associazione Italiana Di Ingegneria Chimica.

Elicitor effects on Penicillium chrysogenum morphology in submerged cultures
Radman, R., Bucke, C. and Keshavarz, T. 2004. Elicitor effects on Penicillium chrysogenum morphology in submerged cultures. Biotechnology and Applied Biochemistry. 40 (3), pp. 229-233. https://doi.org/10.1042/BA20040062

Morphological changes in the submerged cultures of Penicillium chrysogenum: elicitor effects
Radman, R., Bucke, C. and Keshavarz, T. 2004. Morphological changes in the submerged cultures of Penicillium chrysogenum: elicitor effects. in: Pierucci, S. (ed.) 6th Italian Conference on Chemical and Process Engineering: ICheaP 6, June 8-11, 2003, Pisa, Italy Italy Associazione Italiana Di Ingegneria Chimica.

Elicitor effects on reactive oxygen species in liquid cultures of Penicillium chrysogenum
Radman, R., Bucke, C. and Keshavarz, T. 2004. Elicitor effects on reactive oxygen species in liquid cultures of Penicillium chrysogenum. Biotechnology Letters. 26 (2), pp. 147-152. https://doi.org/10.1023/B:BILE.0000012897.28356.cb

Elicitation of plants and microbial cell systems
Radman, R., Saez, T., Bucke, C. and Keshavarz, T. 2003. Elicitation of plants and microbial cell systems. Biotechnology and Applied Biochemistry. 37 (1), pp. 91-102.

Permalink - https://westminsterresearch.westminster.ac.uk/item/93y1q/study-of-the-effects-of-oligosaccharides-in-liquid-cultures-of-penicillium-chrysogenum


Share this

Usage statistics

116 total views
144 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.