Enhanced intracellular Ca(2+) concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors

Murphy, Tania, Nilsson, Asa, Roy, Ipsita, Harrop, Antony, Dixon, Keith and Keshavarz, Tajalli (2011) Enhanced intracellular Ca(2+) concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors. Biotechnology Letters, 33 (5). pp. 985-991. ISSN 0141-5492

Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s10529-010-0511-6

Abstract

Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca(2+) levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca(2+) levels. The apoaequorin gene was cloned into Escherichia coli DH5? and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca(2+) in E. coli and tenfold increase in B. subtilis. These increases in Ca(2+) levels could therefore contribute to the enhancement of secondary metabolite levels.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Miss Nina Watts
Date Deposited: 19 Jan 2011 09:59
Last Modified: 11 Jul 2011 13:48
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/8985

Actions (login required)

Edit Item (Repository staff only) Edit Item (Repository staff only)