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Enhanced intracellular Ca(2+) concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors

Murphy, Tania and Nilsson, Asa and Roy, Ipsita and Harrop, Antony and Dixon, Keith and Keshavarz, Tajalli (2011) Enhanced intracellular Ca(2+) concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors. Biotechnology Letters, 33 (5). pp. 985-991. ISSN 0141-5492

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Official URL: http://dx.doi.org/10.1007/s10529-010-0511-6

Abstract

Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca(2+) levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca(2+) levels. The apoaequorin gene was cloned into Escherichia coli DH5α and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca(2+) in E. coli and tenfold increase in B. subtilis. These increases in Ca(2+) levels could therefore contribute to the enhancement of secondary metabolite levels.

Item Type:Article
Research Community:University of Westminster > Life Sciences, School of
ID Code:8985
Deposited On:19 Jan 2011 09:59
Last Modified:11 Jul 2011 14:48

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