Murphy, Tania and Nilsson, Asa and Roy, Ipsita and Harrop, Antony and Dixon, Keith and Keshavarz, Tajalli (2011) Enhanced intracellular Ca(2+) concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors. Biotechnology Letters, 33 (5). pp. 985-991. ISSN 0141-5492
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Official URL: http://dx.doi.org/10.1007/s10529-010-0511-6
Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca(2+) levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca(2+) levels. The apoaequorin gene was cloned into Escherichia coli DH5α and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca(2+) in E. coli and tenfold increase in B. subtilis. These increases in Ca(2+) levels could therefore contribute to the enhancement of secondary metabolite levels.
|Research Community:||University of Westminster > Life Sciences, School of|
|Deposited On:||19 Jan 2011 09:59|
|Last Modified:||11 Jul 2011 14:48|
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