Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1? and HIF2? in rat oviduct and endometrium

Gillies, Robert M. and Robinson, Simon P. and McPhail, Lesley D. and Carter, Nicholas D. and Murray, Joanne F. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1? and HIF2? in rat oviduct and endometrium. Journal of Molecular Histology, 42 (4). pp. 341-354. ISSN 1567-2379

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Official URL: http://dx.doi.org/10.1007/s10735-011-9338-2

Abstract

Hypoxia is thought to be critical in regulating physiological processes within the female reproductive system, including ovulation, composition of the fluid in the oviductal/uterine lumens and ovarian follicle development. This study examined the localisation of exogenous (pimonidazole) and endogenous [hypoxia inducible factor 1? and 2? (HIF1?, -2?), glucose transporter type 1 (GLUT1) and carbonic anhydrase 9 (CAIX)] hypoxia-related antigens within the oviduct and uterus of the rat reproductive tract. The extent to which each endogenous antigen co-compartmentalised with pimonidazole was also assessed. Female Wistar Furth rats (n = 10) were injected intraperitoneally with pimonidazole (60 mg/kg) 1 h prior to death. Reproductive tissues were removed immediately following death and fixed in 4% paraformaldehyde before being embedded in paraffin. Serial sections were cut (6-7 ?m thick) and antigens of interest identified using standard immunohistochemical procedures. The mucosal epithelia of the ampulla, isthmus and uterus were immunopositive for pimonidazole in most sections. Co-compartmentalisation of pimonidazole with HIF1? was only expressed in the mucosa of the uterus whilst co-compartmentalisation with HIF2? was observed in the mucosa of the ampulla, isthmus and uterus. Both GLUT1 and CAIX were co-compartmentalised with pimonidazole in mucosa of the isthmus and uterus. This study confirms that mucosal regions of the rat oviduct and uterus frequently experience severe hypoxia and there are compartment specific variations in expression of endogenous hypoxia-related antigens, including the HIF isoforms. The latter observation may relate to target gene specificity of HIF isoforms or perhaps HIF2?'s responsiveness to non-hypoxic stimuli such as hypoglycaemia independently of HIF1?.

Item Type: Article
Subjects: University of Westminster > Science and Technology > Life Sciences, School of (No longer in use)
Depositing User: Miss Nina Watts
Date Deposited: 07 Jul 2011 14:57
Last Modified: 22 Aug 2011 10:05
URI: http://westminsterresearch.wmin.ac.uk/id/eprint/9547

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