The relationship between isatin and monoamine oxidase-B inhibitory activity in urine

Pang, F.-Y., Hucklebridge, Frank, Forster, G., Tan, K. and Clow, Angela (1996) The relationship between isatin and monoamine oxidase-B inhibitory activity in urine. Stress Medicine, 12 (1). pp. 35-42. ISSN 1532-3005

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We have investigated the correlation between urinary isatin concentration and MAO-B inhibitory activity. A sensitive enzyme-linked immunosorbent assay (ELISA) for the measurement of isatin in urine was developed. There was a strong correlation between isatin concentrations determined by this method and the established GC/MS technique. In two sets of urine samples from healthy individuals (N = 63 and N = 21), we have shown that there is no significant correlation between isatin concentration and MAO-B inhibitory activity (r = ? 0.1013 and ? 0.1597, respectively). Based upon IC50 measures, the concentration of isatin in normal urine (N = 10) was 3– 5 times less than that which could account for the detected MAO-B inhibitory activity. Furthermore, Lineweaver-Burk plots from urine samples (N = 12) compared with isatin showed that the inhibition by isatin was competitive whereas urine samples could elicit either mixed or non-competitive inhibition. We conclude that the MAO-B inhibitory activity in urine cannot be attributed to isatin alone and another inhibitor(s) appears to be present.

Item Type: Article
Subjects: University of Westminster > Social Sciences and Humanities
Depositing User: Rachel Wheelhouse
Date Deposited: 07 Dec 2011 11:59
Last Modified: 07 Dec 2011 11:59

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