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The relationship between isatin and monoamine oxidase-B inhibitory activity in urine

Pang, F.-Y. and Hucklebridge, Frank and Forster, G. and Tan, K. and Clow, Angela (1996) The relationship between isatin and monoamine oxidase-B inhibitory activity in urine. Stress Medicine, 12 (1). pp. 35-42. ISSN 1532-3005

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Official URL: http://dx.doi.org/10.1002/(SICI)1099-1700(199601)1...

Abstract

We have investigated the correlation between urinary isatin concentration and MAO-B inhibitory activity. A sensitive enzyme-linked immunosorbent assay (ELISA) for the measurement of isatin in urine was developed. There was a strong correlation between isatin concentrations determined by this method and the established GC/MS technique. In two sets of urine samples from healthy individuals (N = 63 and N = 21), we have shown that there is no significant correlation between isatin concentration and MAO-B inhibitory activity (r = − 0.1013 and − 0.1597, respectively). Based upon IC50 measures, the concentration of isatin in normal urine (N = 10) was 3– 5 times less than that which could account for the detected MAO-B inhibitory activity. Furthermore, Lineweaver-Burk plots from urine samples (N = 12) compared with isatin showed that the inhibition by isatin was competitive whereas urine samples could elicit either mixed or non-competitive inhibition. We conclude that the MAO-B inhibitory activity in urine cannot be attributed to isatin alone and another inhibitor(s) appears to be present.

Item Type:Article
Research Community:University of Westminster > Social Sciences, Humanities and Languages, School of
ID Code:9947
Deposited On:07 Dec 2011 11:59
Last Modified:07 Dec 2011 11:59

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